OBJECTIVE:Tovalidatephotographicbioimagingforevaluatingtheseverityofherpessimplexviruskeratitis.METHODS:StromalkeratitisofpatientsintheHerpeticEyeDiseaseStudywasclinicallymeasuredwithaslitbeammicrometerandthenphotographedattrialentry.Calibratedimagesof169eyeswereanalyzedforthesize,location,anddensityofstromalkeratitisandendotheliitis,withshapefactorasafunctionofareaandperimeter.Validitywasassessedbycomparingclinicalandcomputerizedmeasurementsandbycorrelatingthekeratitisareawithvisualacuity.Logisticregressionexploredcharacteristicsassociatedwithlargerordensercornealinflammation.RESULTS:Stromalkeratitishadamedianareaof22.4mm（2）（interquartilerange,12.8-31.6mm（2））withamedianshapefactorof0.69（interquartilerange,0.56-0.79）;126eyes（75%）hadtheirmidpointwithin2mmofthecornea'sgeometriccenter.Photoanalyticalareaestimatesofherpeticstromalkeratitiscorrelatedcloselywithclinicalmeasurements（correlationcoefficient,0.83）.Eyeswithlargerstromalkeratitishadworsevision（correlationcoefficient,0.32）andweremorelikelytohaveiritis（P=.01）.Necrotizingstromalkeratitiswassignificantlywhiter（P=.02）.CONCLUSIONS:Imageanalysisvalidlyassessesthedisciformgeometryofherpeticstromalkeratitisandconfirmsthatincreasedseverityisassociatedwithuveitisandreducedvision.
目的：验证生物摄影图像对单纯疱疹病毒性角膜炎严重性的评价作用。方法：在试验登记时，对疱疹性眼病研究中的基质性角膜炎患者以裂隙光线测微计进行临床测量而后拍摄照片。对169幅眼睛校准图像的基质角膜炎和内皮炎的大小、位置和密度进行分析，以形状因素作为面积和周长的函数。通过临床比较和计算机测量结果以及角膜炎范围与视力的相关性对有效性进行评估。以Logistic回归探究与较大或密度较高角膜炎有关的特征。结果：基质性角膜炎范围的中位数为22.4mm（2）（四分位间距12.8-31.6mm（2）），形状因素的中位数为0.69（四分位间距，0.56-0.79）;126只眼睛（75％）的中点在眼角膜几何中心的2mm内。单纯疱疹性基质性角膜炎的图形分析范围评估与临床测量密切相关（相关系数0.83）。患较大基质性角膜炎的眼睛视力较差（相关系数0.32），且并发虹膜炎的可能性更大（P=0.01）。坏死性基质性角膜炎显著更白（P=0.02）。结论：图像分析有效地评估了单纯疱疹性基质性角膜炎的椭圆几何图，并证实增加的严重性与葡萄膜炎及视力下降有关。
