【详细说明】
规格：1ml3ml0.1ml（浓缩）0.2ml（浓缩）阳性对照（片）试剂名：视网膜母细胞瘤抑制蛋白试剂名（英）：RetinoblastomaSuppressorProtein产品编号：HM-0461阳性部位：细胞核鼠抗人：单克隆抗体阳性对照：正常结肠/乳腺癌克隆号：1F8（Rb1）效价：1：50-1：100预处理：EDTA应用：冰冻/石蜡
Retinoblastoma（Rb）SuppressorProtein
MouseMonoclonalAntibody
Cat.No.HM-0461
SpecificityａndComments:
Recognizesa105kDaphosphoprotein,identifiedasretinoblastoma（Rb）geneproduct.Itsepitopeislocalizedbetweenaa703-772.Itshowsnocrossreactionwithp107orp130.ItspecificallystainsthenucleiofBT-20cellsａndprimaryhumanforeskinfibroblast（HFF）cells.ItdoesnotstaintheRb-negativeBT549cells.This1F8/Rb1antibodyreactswiththehyperphosphorylatedaswellastheun（under）phosphorylatedformoftheRbprotein.InWesternblotting,1F8/Rb1MabdetectedRbproteinin15%ofleukemiasampleswhichwereotherwisetestednegativewithanotherantibodytoRb（Clone3C8）.
SpeciesReactivity:Human.Doesnotreactwithmouseａndrat.Others-notknown.
CloneDesignation:1F8（alsoknownasRb1）
IgIsotype:IgG1
Immunogen:Rb-$-Galactosidasefusionprotein.
Hybridoma:ProducedbyfusionbetweenBALB/csplenocytesａndmousemyelomaSp2/0Ag14cells.
SuppliedAs:200ug/mlantibodypurifiedfromtheascitesfluidbyProteinGchromatography.Preparedin10mMPBS,pH7.4,with0.2%BSAａnd15mMsodiumazide.
KnownApplicationsａndSuggestedWorkingDilutions:
Immunohistology（Frozen&formalin/paraffin）（1:50-1:100for60minuteatRT）
*（Stainingofformalin-fixedtissuesREQUIRESboilingtissuesectionsin1mMEDTA,pH8.0for10-20minfollowedbycoolingatRTfor20min.）
Theoptimaldilutionforaspecificapplicationunderagivensetofexperimentalconditionsshouldbedeterminedbytheinvestigator.
PositiveControl:ML-1,U2OS,orMolt-4cells.Normalcolon.About80%ofbreastcarcinomascontainvaryinglevelsofRbprotein.Useintermixednormalstromalnucleiasinternalpositivecontrol.
AlterationsinRBgeneresultineitherdecreasedorcompletelossofexpressionoftheRbprotein.
CellularLocalization:Nuclear.
StorageａndStability:Storevialat4/C.
ImportantNote:Everytimebeforeyouopenthevial,werecommendbrieflycentrifugingthevialonatabletopcentrifugetodislodgeanyliquidinthecontainer＇scapbecausesmallvolumesofantibodywilloccasionallybecomeentrappedinthesealoftheproductvialduringshipmentａndstorage.
