【详细说明】
规格：1ml3ml0.1ml（浓缩）0.2ml（浓缩）阳性对照（片）试剂名：肾胚胎性恶性混合瘤试剂名（英）：Wilm’sTumorProtein（WT1）产品编号：HM-0526阳性部位：细胞核鼠抗人：单克隆抗体阳性对照：Wilm?s瘤克隆号：WLM04效价：1：25-1：50预处理：柠檬酸应用：冰冻/石蜡
Wilm＇sTumorProtein（WT1）
MouseMonoclonalAntibody
Cat.No.HM-0526
SpecificityａndComments:
Recognizesa47-55kDatumorsuppressorprotein,identifiedasWilm＇sTumor（WT）protein.EpitopeofAb-4mapsbetweenaa1-318.WT,asporadicａndfamilialchildhoodkidneytumor,isgeneticallyheterogeneous.Wilms＇tumorisassociatedwithmutationsofWT1,azinc-fingertranscriptionfactorthatisessentialforthedevelopmentofthemetanephrickidneyａndtheurogenitalsystem.TheWT1geneisnormallyexpressedinfetalkidneyａndmesothelium,ａnditsexpressionhasbeensuggestedasamarkerforWilmstumorａndmesothelioma.Thisantibodyisexcellentforstainingofformalin-fixed,paraffin-embeddedtissues.
SpeciesReactivity:Human.Othersnot-tested.
CloneDesignation:WLM04
IgIsotype/LightChain:IgG1/6.
Immunogen:RecombinanthumanWT1proteincorrespondingtoaa1-318.
Hybridoma:ProducedbyfusionbetweenBALB/csplenocytesａndmyelomaNS1cells.
SuppliedAs:200ug/mlofantibodypurifiedfromascitesfluidbyProteinGchromatography.Preparedin10mMPBS,pH7.4,with0.2%BSAａnd15mMsodiumazide.
ApplicationsａndSuggestedDilutions:
Immunohistology（Frozen&formalin/paraffin）（UseAbat1:25-1:50for60minatRT）
*[Stainingofformalin-fixedtissuesREQUIRESboilingtissuesectionsin10mMcitratebuffer,pH6.0,for10-20minfollowedbycoolingatRTfor20min.]
Theoptimaldilutionforaspecificapplicationshouldbedeterminedbytheinvestigator.
PositiveControl:SK-NEP-1cellsorWilm＇sTumor.
CellularLocalization:Nuclear.
StorageａndStability:Storevialat4/C.
ImportantNote:Everytimebeforeyouopenthevial,werecommendbrieflycentrifugingthevialonatabletopcentrifugetodislodgeanyliquidinthecontainer＇scapbecausesmallvolumesofantibodywilloccasionallybecomeentrappedinthesealoftheproductvialduringshipmentａndstorage.
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