肾细胞癌
发布时间：2009-2-18产地：美国市场价格：面议浏览次数：14次上海锐聪科技发展有限公司联系人：王军会员类型：经销商
姓名：王军职位：总经理E-MAIL：138_18719453@163.com电话：021-26809995,66980655/56886276传真：021-56886276手机：15921990938,13524933556邮编：200070地址：上海市匣北区共和新路1010号佳和商务大厦801室
产品简介本公司长期经营Elisa试剂盒、实验耗材、仪器、酶、细胞等生物化学试剂。价格实惠，质量有保证。详细价格请咨询在线人员。产品详细信息RenalCellCarcinomaMarker（gp200）
MouseMonoclonalAntibody
Cat.No.RM-0460
SpecificityａndComments:
Recognizesa~200kDaglycoprotein（gp200）whichisidentifiedasarenalcellcarcinomamarker.Itsepitoperesidesinthecarbohydratedomainofgp200.Itshowsnosignificantcross-reactivitywithothercarbohydratedeterminants,suchastheLewisbloodgroupantigens,epithelialmembraneantigen,HMFG,ａndABbloodgroupantigens.Innormalkidney,gp200islocalizedalongthebrushborderoftheparsconvolutaａndparsrectasegmentsoftheproximaltubule,aswellasfocallyalongtheluminalsurfaceofBowman＇scapsuleadjoiningtheoutgoingproximaltubule.Ofothernormaltissuesexamined,thegp200isalsolocalizedalongtheluminalsurfacesofbreastlobulesａndducts,theluminalsurfaceoftheepididymaltubularepithelium,withinthecytoplasmofparathyroidparenchymalcells,ａndfocallywithinthecolloidofthyroidfollicles.Thirty-oneothernormaltissuesfailedtoexpresssimilarorcross-reactingantigens.Reportedly,gp200isexpressedby93%ofprimaryａnd84%ofmetastaticrenalcellcarcinomas.Thisantibodymaybeausefulreagentintheinvestigationsofcarcinomasofproximalnephrogenicdifferentiationespeciallythoseshowingtubulardifferentiation.Absenceofreactivitywithalphafetoprotein（AFP）ａndalpha-1-antitrypsinantibodiesａndexclusionofmorphologicalorclinicalcriteriawhichmightotherwiseindicatemetastaticbreastorembryonalcarcinomawouldbefurtherconfirmator.
SpeciesReactivity:HumanａndRat.Others-notknown.
CloneDesignation:66.4.C2
IgIsotype/LightChain:IgG2b/6
Immunogen:Microsomalfractionofhumanrenalcorticaltissuehomogenate.
Hybridoma:ProducedbyfusionbetweenBALB/cmousesplenocytesａndmyelomaSP2/OAg14cells.
SuppliedAs:Tissueculturesupernatantwith15mMsodiumazide.
ApplicationsａndSuggestedDilutions:
Immunohistology（Frozenａndformalin/paraffin）（UseAbat1:25-1:50for60minatRT）
*（Immunohistochemicalstainingofformalin/paraffintissuesisenhancedbydigestionoftissuesectionswithtrypsinat1mg/mlPBSfor15minatRT）
Theoptimaldilutionshouldbedeterminedbytheinvestigator.
PositiveControl:Normalkidneyorrenalcellcarcinoma.
CellularLocalization:Cellmembrane/cytoplasmic.
StorageａndStability:Storevialat4/C.
ImportantNote:Everytimebeforeyouopenthevial,werecommendbrieflycentrifugingthevialonatabletopcentrifugetodislodgeanyliquidinthecontainer＇scapbecausesmallvolumesofantibodywilloccasionallybecomeentrappedinthesealoftheproductvialduringshipmentａndstorage.
