【详细说明】
平滑肌的主要功能是负责空腔脏器的收缩，如血管、胃肠道、膀胱以及子宫等器官。它的结构与骨骼肌有很大的不同。人食管壁包括三层，外纵形和内环形，中间肌层是黏膜层。人食管的蠕动是由平滑
肌收缩严格控制。平滑肌的收缩可能是通过两条不同的途径激活磷脂酶（增加细胞内Ca2+浓度和激活G蛋白）并激活PKC-epsilon依赖的信号通路。在体外培养研究表明人食管平滑肌细胞可以表达Na（+）通
人食道平滑肌细胞（HESMC）提取于人食道组织，第二代冻存。每管含有细胞数>5×105cells/ml，此细胞通过对alpha-smoothmuscleactin和desmin免疫荧光染色验证，经测试不含有HIV-1、HBV
Smoothmuscleisresponsibleforthecontractilityofholloworgans,suchasbloodvessels,thegastrointestinaltract,thebladder,ａndtheuterus.Itsstructurediffers
greatlyfromthatofskeletalmuscle.Thehumanesophaguscontainsthreelayersofmuscleinitswalls,theouterlongitudinalａndinnercircularlayersofthemainmuscular
coatａndthemuscularlayerofthemucosa.Visceralsmoothmusclecellsmakesupallthreelayersalongtheentireorganexceptforthemostrostralfewcentimeters.Smooth
musclecontractioniscriticaltoperistalsisinthehumanesophagusａndthecontractionmaybemediatedbyactivationofphospholipasethroughtwodistinctmechanisms
（increasedintracellularCa2+ａndGproteinactivation）ａndactivatingPKCepsilon-dependentmechanisms[1].Invitrostudyalsoshowsthatculturedhumanesophagealsmooth
musclecellsexpressvoltage-dependentNa（+）channels[2].
HESMCfromScienCellResearchLaboratoriesareisolatedfromhumanesophagus.HESMCarecryopreservedatsecondarycultureａnddeliveredfrozen.Eachvialcontains>5x105
cellsin1mlvolume.HESMCarecharacterizedbyimmunofluorescentmethodwithantibodiestoalpha-smoothmuscleactinａnddesmin.HESMCarenegativeforHIV-1,HBV,HCV,
mycoplasma,bacteria,yeastａndfungi.HESMCareguaranteedtofurtherexpandfor15populationdoublingsattheconditionprovidedbyScienCellResearchLaboratories.
Reference[1].Cao,W.,Chen,Q.,Sohn,U.D.,Kim,N.,Kirber,M.T.,Harnett,K.M.,Behar,J.,Biancani,P.（2001）Ca2+-inducedcontractionofcatesophagealcircularsmoothmuscle
cells.AmJPhysiolCellPhysiol.280（4）:C980-92.[2].Deshpande,M.A.,Wang,J.,Preiksaitis,H.G.,Laurier,L.G.,Sims,S.M.（2002）Characterizationofavoltage-dependentNa（+）currentinhumanesophagealsmoothmuscle.
AmJPhysiolCellPhysiol.283（4）:C1045-55.
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