About10-15%ofpatientswithcervicalcancersufferfromrecurrencedespitehistologicallynegativelymphnodes（pN0）.Occultmicrometastasesorsmalltumourcellclustersmaycontributetodiseaseoutcome.TheaimofthisstudywastocompareattheRNAlevel2knowntumour-associatedgenes,HPV16-E6andcytokeratin19（CK19）,asmolecularmarkersforthedetectionofdisseminatedtumourcells.Real-timereversetranscriptionPCRtechnologywasusedtoquantifygeneexpressioninhistologicallypositiveandnegativesentinellymphnodes（SLN）from70patientswithcervicalcancer.Lymphnodesfromnoncancerpatientswereusedascontrols.Calculatedcopynumberswerenormalisedtothegeometricaverageofthemoststablehousekeepinggenes.Weobservedagoodcorrelation（R=0.915）betweentheexpressionofbothmarkersinSLNwithhistologicallyconfirmedmetastases.However,markergeneexpressiondifferedconsiderablyinhistologicallynegativenodes:CK19transcriptsweredetectedin90of112SLN（80.4%）,whereasonly38nodes（33.9%）werepositiveforHPV16E6mRNA.Inparticular,62of74SLN,whichwerenegativebyhistology,andHPV16E6mRNAexpressedCK19mRNA.Moreover,8of10lymphnodesfromnoncancerpatientsexpressedCK19mRNA.SystematicerrorsduetoRNAdegradationorincompletecDNAcouldberuledout.ItisconcludedthatHPV16E6mRNAismorespecificandmoresensitiveforthedetectionoftumourcellsinSLNthanCK19mRNA.ThespecificityofCK19islimitedbecauseoflowlevelexpressioninuninvolvedpelviclymphnodes.©2007Wiley-Liss,Inc.
Received:9October2006;Accepted:6November2006
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