前言研究了姜片吸虫成虫冷浸抗原检测姜片吸虫病患者血清抗体的敏感性和特异性及其在流行病和临床上的应用价值。超声粉碎制备姜片吸虫成虫冷浸抗原。以评价ELISA法检测姜片虫病血清抗体的敏感性、特异性和交叉反应性。按常规ELISA方法操作,目视与酶标仪判断结果。普查2189人中姜片吸虫卵阳性者168例;168例中165例ELISA阳性,ELISA与改良加藤法的阳性符合率98。21%;健康居民血清147份,阳性6份,阳性率4。08%;与血吸虫病交叉阳性为9。38%,肺吸虫病交叉阳性为5。36%。姜片吸虫成虫冷浸抗原1∶3500工作浓度包被酶标板,检测姜片虫病血清抗体具有敏感性高、特异强和交叉反应率低的特点。
Thispaperstudiedsensitivity,specificityandpracticalvalueandsignificanceofELISAinthedetectionofantibodiesagainstFasciolopsisbuskiinhumansera.168casesofFasciolopsiasisweredetectedforantibodiesagainstFasciolopsisbuskiinserabyELISA,andacomparativeinvestigationwasdoneinthelocalresidentswithoutFasciolopsisbuskiand96casesofSchistosomiasisjaponicaand56casesofParagonimuswestermanibyELISAforassessmentofsensitivity,specificityandcrossingreaction....
