前言目的:探讨原发与复发星形胶质细胞瘤中端粒酶活性情况。方法:采用TRAP银染法。结果:Ⅲ级及其以上恶性星形胶质细胞瘤中端粒酶活性明显高于Ⅱ级及以下胶质瘤(1/17,5。9%)(P<0。05);2年内复发的胶质瘤总的阳性率(8/11,72。7%)明显高于原发胶质瘤的阳性率(8/29,27。6%)(X2=5。02,P<0。05),原发、复发胶质瘤的差别主要分布在Ⅰ-Ⅱ级星形胶质细胞瘤中。结论:端粒酶可能是促进星形胶质细胞瘤复发及恶化的主要因素之一。
Aim:Toevaluatethetelomeraseactivityinprimaryandsecondaryastrocytomas.Methods:TRAP(TelomericRepeatAmplificationProtocol)-silverstainingwasusedtodetecttelomeraseactivity.Results:Thepositiverateofastrocytomas(Grad≥Ⅲ,7/12,58.3%)isobviouslyhigherthantherateofastrocytomas(Grad≤Ⅱ,1/17,5.9%)(P<0.05).Thepositiverateofsecondaryastrocytomaswithin2yearsisobviouslyhigherthantherateofprimaryghomasandthedistributionisdifferentinGradeⅠ-Ⅱbetween...